Application

Yeast

Label-free imaging of unstained yeast — no dyes, no fixation, no fluorescent reporters.

One measurement, four contrast modes

Brightfield for morphology, quantitative phase for dry mass, DIC for fine relief, scattering angle for boundaries and texture — all from one fast, automated measurement, with every cell in the field of view captured at once.

Brightfield image of unstained S. cerevisiae yeast cells
Brightfield
Quantitative phase image of the same yeast cells
Quantitative phase
DIC image of the same yeast cells
DIC
Scattering image of the same yeast cells
Scattering

Unstained S. cerevisiae. Scale bar 50 µm.

Dry mass for each cell

With mAIday workflow generator, every cell is segmented and its dry mass read directly from the phase image — no stains, no manual outlining.

Segmented yeast cells on a dark background, each coloured by its dry mass from 4.6 to 60.5 pg

Segmented cells, coloured by dry mass.

Scatter plot of dry mass against diameter for each cell, rising from about 5 pg at 2 µm to about 60 pg at 8 µm

Dry mass vs diameter.

Histogram of dry cell mass, with most cells between 15 and 30 pg

Dry mass distribution (pg).

Also suited to

Organoids · C. elegans · Spheroids · 2D and 3D cell culture · Biocondensates